Fig_6A,B_IFA Version 1
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RIG-I KO IFN response cumulative.pzfx
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Created: 2nd Jan 2024 at 17:24
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Version 1 (earliest) Created 2nd Jan 2024 at 17:24 by John Cornelius
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https://orcid.org/0009-0003-6381-9603
Public web page: Not specified
Submitter: John Cornelius
Studies: Viral Protein Accumulation of Zika Virus Variants Links with Regulation ...
Assays: Bioinformatics analyses of NanoString data, Deep sequencing of ZIKV stocks and sequence alignment, FFU assay for determining the titers of ZIKV stocks, FFU assay for measuring viral spread, Flow cytometry, Immunoblot analysis, Immunofluorescence analysis, Plaque assay, Protein lysate quantification, Quantification of extracellular and intracellular RNA, Quantification of extracellular and intracellular virions, Sample - Metadata, Transcript analysis by NanoString, Transcript analysis by SYBR green qRT-PCR, Viral copy number quantification by TaqMan qRT-PCR, ZIKV attachment and entry assay, ZIKV infection, ZV-13 antibody purification and quantification
Asian lineage Zika virus (ZIKV) strains emerged globally, causing outbreaks linked with critical clinical disease outcomes unless the virus is effectively restricted by host immunity. We have previously shown that retinoic acid-inducible gene-I (RIG-I) senses ZIKV to trigger innate immunity to direct interferon (IFN) production and antiviral responses that can control ZIKV infection. However, ZIKV proteins have been demonstrated to antagonize IFN. Here, we conducted in vitro analyses to assess ...
Submitter: John Cornelius
Investigation: Zika Virus
Assays: Bioinformatics analyses of NanoString data, Deep sequencing of ZIKV stocks and sequence alignment, FFU assay for determining the titers of ZIKV stocks, FFU assay for measuring viral spread, Flow cytometry, Immunoblot analysis, Immunofluorescence analysis, Plaque assay, Protein lysate quantification, Quantification of extracellular and intracellular RNA, Quantification of extracellular and intracellular virions, Sample - Metadata, Transcript analysis by NanoString, Transcript analysis by SYBR green qRT-PCR, Viral copy number quantification by TaqMan qRT-PCR, ZIKV attachment and entry assay, ZIKV infection, ZV-13 antibody purification and quantification
A549 cells were seeded on no. 1 glass coverslips in a 24-well plate at a density of 1 × 105 cells per well. The next day, cells were mock infected or infected with ZIKV strains and fixed with 4% formaldehyde (Fisher Scientific) in filtered 1× TBS for 30 min at room temperature. Cells were then washed with filtered 1× TBS and permeabilized and blocked in 5% NGS in 1× TBS (5% NGS/TBS) containing 0.1% Triton X-100 for 30 min at 4°C. For pSTAT1 staining, cells were permeabilized with 100% ice-cold ...
Submitter: John Cornelius
Assay type: Immunoassay
Technology type: Microscopy
Investigation: Zika Virus
