A549 cells were infected as described above. At the appropriate time points, 1 mL of cell culture supernatants was harvested and centrifuged at 2,000 rpm for 10 min at 4°C. Extracellular RNA was extracted from the clarified supernatants using the QIAmp viral RNA minikit to quantify extracellular viral copy numbers. Cells were washed with ice-cold stringent wash buffer (1 M NaCl, 50 mM sodium bicarbonate, pH 9.5) for 3 min to remove cell surface-associated viruses. After ice-cold 1× PBS washes, cells were harvested using the Qiagen RNeasy kit to extract intracellular RNA to quantify intracellular viral copy numbers.
SEEK ID: https://seek.galelab.org/assays/7
Experimental assay
Projects: Zika Virus Investigation
Investigation: Zika Virus
Assay position:
Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: No organisms
Creator
Submitter
Views: 62
Created: 14th Nov 2023 at 22:04
Last updated: 3rd Jan 2024 at 17:38
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