Vero cells were seeded at a density of 1.85 × 105 cells per well in 6-well plates. The next day, supernatants from infected samples were serially diluted in DMEM containing 1% FBS. Vero cells were incubated with dilutions in duplicate at 37°C for 1 h with rocking. Following incubation, cells were overlaid with 1% low-melt agarose (Fisher Scientific), 5% sodium bicarbonate (Fisher Scientific), and 5% FBS in 2× DMEM (Fisher Scientific). Plaques were visualized 4 to 5 days later with a 1% low-melt agarose overlay containing 3% neutral red (Sigma-Aldrich), 10% 10× PBS (Fisher Scientific), and dH2O.
SEEK ID: https://seek.galelab.org/assays/9
Experimental assay
Projects: Zika Virus Investigation
Investigation: Zika Virus
Assay position:
Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: No organisms
Creator
Submitter
Views: 70
Created: 14th Nov 2023 at 23:33
Last updated: 3rd Jan 2024 at 17:38
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