Fig_6E-I_Flow_Cytometry Version 1
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RIG-I KO IFN response NS5 linear regression cumulative.pzfx
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Created: 2nd Jan 2024 at 17:20
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Version 1 (earliest) Created 2nd Jan 2024 at 17:20 by John Cornelius
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https://orcid.org/0009-0003-6381-9603
Public web page: Not specified
Submitter: John Cornelius
Studies: Viral Protein Accumulation of Zika Virus Variants Links with Regulation ...
Assays: Bioinformatics analyses of NanoString data, Deep sequencing of ZIKV stocks and sequence alignment, FFU assay for determining the titers of ZIKV stocks, FFU assay for measuring viral spread, Flow cytometry, Immunoblot analysis, Immunofluorescence analysis, Plaque assay, Protein lysate quantification, Quantification of extracellular and intracellular RNA, Quantification of extracellular and intracellular virions, Sample - Metadata, Transcript analysis by NanoString, Transcript analysis by SYBR green qRT-PCR, Viral copy number quantification by TaqMan qRT-PCR, ZIKV attachment and entry assay, ZIKV infection, ZV-13 antibody purification and quantification
Asian lineage Zika virus (ZIKV) strains emerged globally, causing outbreaks linked with critical clinical disease outcomes unless the virus is effectively restricted by host immunity. We have previously shown that retinoic acid-inducible gene-I (RIG-I) senses ZIKV to trigger innate immunity to direct interferon (IFN) production and antiviral responses that can control ZIKV infection. However, ZIKV proteins have been demonstrated to antagonize IFN. Here, we conducted in vitro analyses to assess ...
Submitter: John Cornelius
Investigation: Zika Virus
Assays: Bioinformatics analyses of NanoString data, Deep sequencing of ZIKV stocks and sequence alignment, FFU assay for determining the titers of ZIKV stocks, FFU assay for measuring viral spread, Flow cytometry, Immunoblot analysis, Immunofluorescence analysis, Plaque assay, Protein lysate quantification, Quantification of extracellular and intracellular RNA, Quantification of extracellular and intracellular virions, Sample - Metadata, Transcript analysis by NanoString, Transcript analysis by SYBR green qRT-PCR, Viral copy number quantification by TaqMan qRT-PCR, ZIKV attachment and entry assay, ZIKV infection, ZV-13 antibody purification and quantification
Cells were seeded, infected, or transfected and treated with IFN-β as described above. Following IFN-β treatment, A549 RIG-I KO and HEK293T cells were pelleted and fixed with 4% formaldehyde in 1× PBS at room temperature for 15 min. Cells were then washed with excess 1× PBS, pelleted, and resuspended in 1× PBS for storage at 4°C. Fixed cells were permeabilized with 90% ice-cold methanol in 1× PBS for at least 10 min on ice. Cells were washed with excess 1× PBS and incubated for 1 h at room ...
Submitter: John Cornelius
Assay type: Experimental Assay Type
Technology type: Flow Cytometry
Investigation: Zika Virus
