Assays
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Created At
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Project
Zika Virus Investigation1
Submitter
John Cornelius1
Creator
John Cornelius1
Assay class
Experimental assay1
Assay type
Experimental Assay Type1
Technology type
Technology Type1
1
Assay visible to you, out of a total of 1
Vero cells were seeded at a density of 1.85 × 105 cells per well in 6-well plates. The next day, supernatants from infected samples were serially diluted in DMEM containing 1% FBS. Vero cells were incubated with dilutions in duplicate at 37°C for 1 h with rocking. Following incubation, cells were overlaid with 1% low-melt agarose (Fisher Scientific), 5% sodium bicarbonate (Fisher Scientific), and 5% FBS in 2× DMEM (Fisher Scientific). Plaques were visualized 4 to 5 days later with a 1% low-melt ...
Submitter: John Cornelius
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Zika Virus