Assays
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Created At
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Project
Zika Virus Investigation1
Submitter
John Cornelius1
Creator
John Cornelius1
Assay class
Experimental assay1
Assay type
Experimental Assay Type1
Cells were seeded, infected, or transfected and treated with IFN-β as described above. Following IFN-β treatment, A549 RIG-I KO and HEK293T cells were pelleted and fixed with 4% formaldehyde in 1× PBS at room temperature for 15 min. Cells were then washed with excess 1× PBS, pelleted, and resuspended in 1× PBS for storage at 4°C. Fixed cells were permeabilized with 90% ice-cold methanol in 1× PBS for at least 10 min on ice. Cells were washed with excess 1× PBS and incubated for 1 h at room ...
Submitter: John Cornelius
Assay type: Experimental Assay Type
Technology type: Flow Cytometry
Investigation: Zika Virus