Assays
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Created At
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Project
Zika Virus Investigation1
Submitter
John Cornelius1
Creator
John Cornelius1
Assay class
Experimental assay1
Assay type
Experimental Assay Type1
Technology type
Technology Type1
1
Assay visible to you, out of a total of 1
A549 cells were infected as described above. At the appropriate time points, 1 mL of cell culture supernatants was harvested and centrifuged at 2,000 rpm for 10 min at 4°C. The clarified supernatants were used for plaque assays to quantify extracellular virions. Cells were washed with ice-cold stringent wash buffer as described above and then detached with 0.05% trypsin-EDTA (Fisher Scientific). Cells were resuspended in 1 mL cDMEM and centrifuged at 300 × g for 5 min at 4°C. Cell pellets were ...
Submitter: John Cornelius
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Zika Virus