Assays
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Project
Zika Virus Investigation1
Submitter
John Cornelius1
Creator
John Cornelius1
Assay class
Experimental assay1
Assay type
Immunoassay1
Hybridoma supernatant was harvested as the source of ZV-13 antibody once ~95% cell death was observed. The supernatant was clarified by centrifuging for 10 min at 400 × g at 4°C and then filtered with a 0.22-μm filter prior to antibody purification via protein A affinity chromatography using the Pierce Protein A IgG purification kit and NAb Protein A Plus spin columns (all from Thermo Fisher-Life Technologies). Purified ZV-13 antibody was concentrated and buffer-exchanged into 1× phosphate-buffered ...
Submitter: John Cornelius
Assay type: Immunoassay
Technology type: Affinity Chromatography
Investigation: Zika Virus