Assays

RNA samples were diluted to a final concentration of 20 ng/μL and hybridized to reporters and capture probes from a custom-generated innate immunity probe set (NanoString, Seattle, WA, USA). Following hybridization for 16 h at 65°C, sample preparation was completed on an nCounter MAX prep station (NanoString) according to the manufacturer’s protocol. RNA transcript counts were quantified on a NanoString nCounter (NanoString) according to the manufacturer’s instructions.